Responsibility for implementation of the gender mainstreaming strategy lies with the senior management in each United Nations entity, as clearly stated in the Letter from the Secretary-General to heads of all United Nations entities in October 1997. In many parts of the United Nations system Gender Advisor posts have been established to support management to undertake their roles in implementing gender mainstreaming.

Gender advisors promote and support gender-sensitive approaches to policy and program work within a given mission, office, team, etc. They provide strategic advice in planning and policy making processes, in coordination meetings and task forces, as well as through existing gender units or gender focal points. They may be responsible for strategies such as: advocacy and awareness raising; training and capacity building; monitoring and advising; evaluation and reporting; and technical advice and support. Their work often focuses as much on in-house operations as it does liaising with national and regional partners to ensure that gender issues are adequately addressed.